Effect of Chloroform Inhalation on the Hippocampus of Adult Male Wistar Rat

U.S. Aguwa, F.O. Ovie, S.I. Olu, O. Ukoba

Abstract


Chemicals used in laboratories may pose health threats to individuals handing them. This work was carried out to evaluate the effect of chloroform inhalation on the hippocampus of Wistar rats. Fourteen male Wistar rats weighing 150-180g were divided into two groups of 7 rats each. Group A was the control group and Group B was the experimental group which was exposed to 50ml of chloroform for 5minutes daily in an inhalation chamber for 14 days. Neurobehavioral study was done before and after exposure and the rats were sacrificed. Two rat brains were fixed in Bouin’s fluid for histological studies and the other five for antioxidant studies. Results were analyzed using SPSS and values were significant at P≤0.05. Results reveal the animals in group B were under oxidative stress compared to those in control. Although rat weight increased in the 2 groups, the percentage increase was lower in the experimental group B compared to control. Histological analysis showed varying degrees of necrosis in the hippocampus of group B rats. Neurobehavioral studies revealed significant increase in the time to discover the escape platform compared to control. We therefore infer that chloroform inhalation has neurodegenerative effects on the hippocampus of Wistar rats.

Key words: hippocampus, chloroform, neurobehavioral, inhalation


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