Effect of Combination of Pure Isolates of Eichhornia Crassipes’ Roots on Bioactivity Against Some Cancer Cell Lines
Abstract
The fight against cancers still demands robust research from every possible angle including phytomedicine to find safer and more effective agents or approaches to therapry. One such approach is combinatory therapy. This work aimed at isolating active compounds from Eichhornia crassipes’ roots that would be tested for activity against MCF-7 and HepG2 cell lines both individually and in combination. UV Chromatograms from a liquid chromatography with a Mass Spectroscopy detector was used to confirm the purity of the isolated compounds. MCF-7 and HepG2 cell lines were subjected to four compounds (1-4) isolated from the roots of Eishhornia crassipes with doxorubicin as a standard. Compound 3 was the most active against MCF-7 with an IC50 of less than 4 ug/ml. When combined in pairs, a combinatory dose of compounds 2 and 3 was the most active against HepG2 (IC50 = 3.1 ± 0.16 ug/ml) while that of compound 3 and 4 had the highest activity against MCF-7 with an IC50 of 3.82 ± 0.06 ug/ml. It was noted that not every combinatory dose has an additive effect. Only 40.6% of the tests showed increased activity, 34.4% showed reduced activity and there was no change in activity in 25% of the tests. Combinatory doses require logical strategies for better clinical outcomes.Keywords: Combination therapy, Drug Resistance, Eichhornia crassipes, Synergy, Antagonistic effect, Purified compounds
Copyright (c) 2020 Andrew G. Mtewa, Duncan Sesaazi
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