Physiological Properties and Medium Optimization of MGPR3.1 Thermo-Alkaliphile Isolate

  • G. Indriati
  • R. R. P. Megahati
  • D. W. Putri


Amylase produced by bacteria or bacteria amylase is an enzyme that can hydrolyze starch to sugar. Amylase-producing bacteria can be found in seawater, volcanic craters, compost, and hot springs. Hot springs are a potential place as a source of amylase-producing bacteria. Indonesia has many hot springs such as Pariangan hot springs. The temperature of Pariangan hot spring between 47-50°C and pH ranged between 8.4-9.0. This caused Pariangan hot springs have a high level of bacterial diversity. Isolation and screening of thermo-alkaliphile amylase producing bacteria originating from Pariangan hot spring have been successfully performed. There were 4 isolates of 1 bacterium isolate with the highest activity, i.e. isolate MGPR3.1. Physiological properties and optimization medium of amylase activity are unknown. The aim of the study was to determine the physiological properties and increase the amylase activity produced by MGPR3.1 isolates. The results showed that MGPR3.1 isolates were Gram-positive and belonged to Bacillus sp.Keywords: physiological properties, optimization, thermo-alkaliphile, hot spring