Molecular Detection of CCR5 D32 Allele Gene among Discordant HIV-Infected Couples in Kaduna State, Nigeria

  • Daniel Abba Danboyi
  • Nura Muhammad Sani
  • Lawal Danjuma
  • Mas’ud Abdullahi
Keywords: Antiretroviral therapy, Discordance, HIV, CCR5 D32, Sexually transmitted infection

Abstract

Discordance is a situation where one partner is HIV positive while the other remains negative within couples and the CCR5 D32 gene is the mutant variant of the CCR5 wt. fusion protein which confers resistance to HIV infection. The objective of the research was to establish the incidence of discordance among HIV-infected couples in Kaduna state and to determine the frequency of the CCR5 D32 mutant allele gene among discordant HIV-infected couples. A total of 158 couples and 317 individuals were recruited for the survey using a stratified sampling technique. The 23 local government areas of Kaduna State were divided into 3 strata (senatorial zones). These respondents accessed treatment at 9 HIV treatment centers in 9 local government areas of the 23 local government areas of Kaduna State. A blood test was conducted to confirm their HIV status and ensure they were discordant couples. Blood samples of negative partners were analyzed using Primers of the CCR5 gene in the molecular laboratory. The results showed that more women 105 (33.12%) were HIV positive, and the HIV discordance prevalence for Kaduna State was 0.2%. The results also showed that the frequency of the CCR5 D32 allele gene in the population was 1.3%, and no correlation between HIV discordance and the CCR5 D32 allele gene. The incidence of the CCR5 D32 gene was 1.3%. It is hereby recommended that more research be conducted in other African countries to ascertain the frequency of the CCR5 D32 allele gene.
Published
2022-03-24
Section
Articles