Effect of Acetylsalicylic Acid on Haematology and Biochemical Indices of Two Strains of Layers

  • O. I. Baki
  • S. O. Aro
  • O. T. Owolabi


This project investigated the effect of acetylsalicylic acid on haematology and serum biochemical indices of two strains of layers. One hundred and ninety two (192) layers comprising ninety six (96) each of Harco Black and Isa Brown layers were used for the experiment that lasted sixteen weeks. Birds were randomly allotted to four treatment groups designated as T1 (control with 0.00% ASA while the remaining three (3) diets were labeled as T2, T3 and T4 contains 0.025%, 0.050% and 0.075% of ASA per 100kg of feed, respectively. Each treatment was replicated four times with twelve (12) birds per replicate. At the end of 16th week of the experiments, bloods from forty eight birds (12 birds per treatment group) were collected through venipuncture of the axillary vein of each bird. About 5ml of blood were dispensed into each of the Ethylene Diamine Tetra Acetic Acid (EDTA) bottles and sterile test tubes. The blood samples in the EDTA bottles were rocked gently so as to enable their mixture with the EDTA in order to prevent coagulation and used to determine haematological indices while those in the sterile test tubes were slanted and allowed to clot after which the sera were harvested for biochemical analyses. Results from blood samples collected at the end of the experiment showed significant breed differences in erythrocyte sedimentary rate, haemoglobin (Hb), red blood cells (RBC) and packed cell volume (PCV) between the IB and HB strain. IB had higher Hb, RBC and PCV than the HB strain. The dietary inclusion of ASA had significant effects on the heterophil and monocyte components of the white blood cell when strain and treatment levels were considered together. Isa Brown had higher serum albumin level than the HB strain. The use of ASA in the diet at 0.075% significantly (p<0.05) lowered the serum cholesterol and Alkaline Phosphatase. The interactions between breed and ASA levels showed significant differences in all biochemical parameters measured. It could however be concluded that the use of ASA in the feed at the levels used in this study did not have any significantly effect on the haematological parameters but had a better ameliorative action on the heterophils of the IB than the HB strain. The use of ASA at 0.075% lowered the serum cholesterol and ALP levels in the birds.Key words: Aspirin, Isa Brown, Harco Black, Haematology, Biochemical Indices