The Effect of Piper betle L. Extract on the Growth of Streptococcus pneumoniae Colonies in Wistar Stand Rats Model Bacterial Conjunctivitis


  • Yudhi Pratama
  • Fithria Aldy


Piper betle L., Streptococcus pnemumonia, conjunctivitis, Total Plate Count


Background. Conjunctivitis is an eye infection accounted as the third leading ophthalmology cases, with Streptococcus pneumoniae as the main causes in children. Several studies had indicated that Piper betle L. extract inhibited Streptococcus pneumoniae growth.

Purpose. To determine the effect of Piper betle L. extract on the growth of Streptococcus pneumoniae colonies in bacterial conjunctivitis of Wistar Rat Model.

Methods. This study was an analytical study with true experimental design and pre-test-post-test control group. Streptococcus pneumoniae colony growth media was analyzed using Mueller Hinton agar and calculated using Total Plate Count methods.

Result. The sample of this study was 30 male wistar rats. Kruskal Wallis test showed a significant difference between Streptococcus pneumoniae colonies based on the treatment group (p=0.023). Posthoc test showed significant differences in Streptococcus pneumoniae colonies between the placebo group and the 25% (p = 0.039) and 30% (p = 0.006) Piper betle group. No significant difference was found between the placebo group and the group using 20% betel (p=0.159) and ofloxacin (p=1,000).

Conclusion. There was a significant difference in Streptococcus pneumoniae colonies between the placebo group and the 25% and 30% betel eye drops group.