Molecular and Morphological Characterization of Wheat Genotypes under Drought Condition in Nigerian Sudan Savannah
DOI:
https://doi.org/10.59573/emsj.7(5).2023.11Ключевые слова:
Wheat genotypes, Drought stress, Genetic diversity, Proline concentration, SSR markersАннотация
Drought stress is one of the most important abiotic constrains affecting cereal crop in the world that causes serious yield loses and threat to sustainability and food security especially in wheat thereby causing the insufficient supply of food. Therefore, understanding the genetic characterization of drought stress response is very important. This study was designed to reveal the morphological and molecular characterization of wheat genotypes using simple sequence repeat (SSR) markers and to estimate the genetic diversity and relationships among the cultivars subjected to drought conditions. A total of 15 wheat genotypes were evaluated for quantitative morphological traits such as plant height, number of seeds per spike, 1000 seed weight, spike length, flag leaf length, grain weight and proline content. Molecular characterization was done using a set of simple sequence repeat (SSR) markers. A total of 12 SSR markers were used to analyze the varieties and the genetic diversity and relationship among them. The results showed that, the drought-stressed plants had lower plant height, number of seeds per spike, 1000 seed weight, spike length, flag leaf length and grain weight than the non-stressed plants, while proline content was found to be higher in stressed plants than the non-stressed. Molecular analyses indicated significant variation among the genotypes with the mean PIC value of 0.64, mean heritability ratio of 0.67 and mean allele number of 8.9. This study also indicates the significance of SSRs as a useful tool in marker-assisted breeding about drought tolerance and for developing strategies for improving drought tolerance in cereals.
Библиографические ссылки
Abdulkerim, J., Tana, T., & Eticha, F. (2015). Response of bread wheat (Triticum aestivum L.) varieties to seeding rates at Kulumsa, South eastern Ethiopia. Asian Journal of Plant Sciences, 14(2), 50–58. https://doi.org/10.3923/AJPS.2015.50.58
Al-Tamimi, A. J. T., & Al-Janabi, A. S. (2019). Genetic diversity among bread wheat genotypes using rapd and ssr markers. Sabrao Journal of Breeding and Genetics, 51(3), 325–399.
Araus, J. L., Slafer, G. A., Reynolds, M. P., & Royo, C. (2002). Plant Breeding and Drought in C3 Cereals: What Should We Breed For? Annals of Botany, 89(7), 925–940. https://doi.org/10.1093/AOB/MCF049
Ates-Sonmezoglu, Ö., Çevik, E., & Terzi-Aksoy, B. (2022). Assessment of some bread wheat (Triticum aestivum L.) genotypes for drought tolerance using SSR and ISSR markers. Biotech Studies, 31(2), 45–52. https://doi.org/10.38042/biotechstudies.1159128
Ateş Sönmezoğlu, Ö., & Terzi, B. (2018). Characterization of some bread wheat genotypes using molecular markers for drought tolerance. Physiology and Molecular Biology of Plants, 24(1), 159–166. https://doi.org/10.1007/s12298-017-0492-1
Chowdhury, M. K., Hasan, M. A., Bahadur, M. M., Islam, M. R., Iqbal, M. A. H. A., Javed, T., Raza, A., Shabbir, R., Sorour, S., Elsanafawy, N. E. M., Anwar, S., Alamri, S., Sabagh, A. EL, & Islam, M. S. (2021). Evaluation of Drought Tolerance of Some Wheat ( Triticum. Agronomy, 11(9), 1–20.
Colasuonno, P., Lozito, M. L., Marcotuli, I., Nigro, D., Giancaspro, A., Mangini, G., De Vita, P., Mastrangelo, A. M., Pecchioni, N., Houston, K., Simeone, R., Gadaleta, A., & Blanco, A. (2017). The carotenoid biosynthetic and catabolic genes in wheat and their association with yellow pigments. BMC Genomics, 18(1), 122. https://doi.org/10.1186/s12864-016-3395-6
Dadbakhsh, A., Yazdansepas, A., & Ahmadizadeh, M. (2011). Study drought stress on yield of wheat (Triticum aestivum L.) genotypes by drought tolerance indices. Advances in Environmental Biology, 5(7), 1804–1810.
EL Sabagh, A., Islam, M. S., Skalicky, M., Ali Raza, M., Singh, K., Anwar Hossain, M., Hossain, A., Mahboob, W., Iqbal, M. A., Ratnasekera, D., Singhal, R. K., Ahmed, S., Kumari, A., Wasaya, A., Sytar, O., Brestic, M., ÇIG, F., Erman, M., Habib Ur Rahman, M., … Arshad, A. (2021). Salinity Stress in Wheat (Triticum aestivum L.) in the Changing Climate: Adaptation and Management Strategies. In Frontiers in Agronomy (Vol. 3, p. 661932). Frontiers Media S.A. https://doi.org/10.3389/fagro.2021.661932
FAO. (2020). China | Global Partnership Initiative for Plant Breeding Capacity Building | Food and Agriculture Organization of the United Nations. http://www.fao.org/in-action/plant-breeding/our-partners/asia/china/en/
Gupta, N. K., Gupta, S., & Kumar, A. (2001). Effect of Water Stress on Physiological Attributes and their Relationship with Growth and Yield of Wheat Cultivars at Different Stages. Journal of Agronomy and Crop Science, 186(1), 55–62. https://doi.org/10.1046/J.1439-037X.2001.00457.X
Kumar, L., Singh, M., & Arora, V. (2020). Influence of Drought Stress on Morphological Characters in Indian Wheat Cultivars. International Journal of Current Microbiology and Applied Sciences, 9(10), 816–832. https://doi.org/10.20546/ijcmas.2020.910.098
Liu, S., Sehgal, S. K., Li, J., Lin, M., Trick, H. N., Yu, J., Gill, B. S., & Bai, G. (2013). Cloning and characterization of a critical regulator for preharvest sprouting in wheat. Genetics, 195(1), 263–273. https://doi.org/10.1534/genetics.113.152330
Mansour, H. A., El Sayed Mohamed, S., & Lightfoot, D. A. (2020). Molecular studies for drought tolerance in some Egyptian wheat genotypes under different irrigation systems. Open Agriculture, 5(1), 280–290. https://doi.org/10.1515/opag-2020-0030
Mkhabela, S. S., Shimelis, H., & Mashilo, J. (2020). Genetic differentiation of selected drought and heat tolerant wheat genotypes using simple sequence repeat markers and agronomic traits. South African Journal of Plant and Soil, 37(3), 211–219. https://doi.org/10.1080/02571862.2020.1718787
Mwadzingeni, L., Shimelis, H., Tesfay, S., & Tsilo, T. J. (2016). Screening of bread wheat genotypes for drought tolerance using phenotypic and proline analyses. Frontiers in Plant Science, 7(AUG2016), 182193. https://doi.org/10.3389/FPLS.2016.01276/BIBTEX
Nagy, S., Poczai, P., Cernák, I., Gorji, A. M., Hegedűs, G., & Taller, J. (2012). PICcalc: An online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics, 50(9–10), 670–672. https://doi.org/10.1007/s10528-012-9509-1
Pour-Aboughadareh, A., Mohammadi, R., Etminan, A., Shooshtari, L., Maleki-Tabrizi, N., & Poczai, P. (2020). Effects of Drought Stress on Some Agronomic and Morpho-Physiological Traits in Durum Wheat Genotypes. Sustainability, 12(14), 5610. https://doi.org/10.3390/SU12145610
Pour-Aboughadareh, A., Omidi, M., Naghavi, M. R., Etminan, A., Mehrabi, A. A., Poczai, P., & Bayat, H. (2019). Effect of Water Deficit Stress on Seedling Biomass and Physio-Chemical Characteristics in Different Species of Wheat Possessing the D Genome. Agronomy, 9(9). https://doi.org/10.3390/AGRONOMY9090522
Qaseem, M. F., Qureshi, R., Shaheen, H., Pakistan, R., & Rawalpindi, P. (2019). Effects of Pre-Anthesis Drought, Heat and Their Combination on the Growth, Yield and Physiology of diverse Wheat (Triticum aestivum L.) Genotypes Varying in Sensitivity to Heat and drought stress. Scientific Reports, 9(1), 1–12. https://doi.org/10.1038/s41598-019-43477-z
Ramadugu, C., Keremane, M. L., Hu, X., Karp, D., Federici, C. T., Kahn, T., Roose, M. L., & Lee, R. F. (2015). Genetic analysis of citron (Citrus medica L.) using simple sequence repeats and single nucleotide polymorphisms. Scientia Horticulturae, 195, 124–137. https://doi.org/10.1016/j.scienta.2015.09.004
Rasheed, A., & Xia, X. (2019). From markers to genome-based breeding in wheat. In Theoretical and Applied Genetics (Vol. 132, Issue 3, pp. 767–784). Springer Verlag. https://doi.org/10.1007/s00122-019-03286-4
Raza, A., Razzaq, A., Mehmood, S. S., Zou, X., Zhang, X., Lv, Y., & Xu, J. (2019). Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review. Plants 2019, Vol. 8, Page 34, 8(2), 34. https://doi.org/10.3390/PLANTS8020034
Sharma, B. R., Mohan, G., Manchanda, S., & Amarasinghe, U. A. (2018). Water Productivity Mapping of Major Indian Crops. October.
Tefera, A., Kebede, M., Tadesse, K., & Getahun, T. (2021). Morphological, Physiological, and Biochemical Characterization of Drought-Tolerant Wheat (Triticum spp.) Varieties. International Journal of Agronomy, 2021. https://doi.org/10.1155/2021/8811749
Yadav, V., Kumar, P., & Goyal, M. (2018). Evaluation of genetic diversity in drought tolerant and sensitive varieties of wheat using ISSR markers. Electronic Journal of Plant Breeding, 9(1), 146–153. https://doi.org/10.5958/0975-928X.2018.00017.0
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