Molecular Characterisation of Anopheles Species Collected from Two Area Councils of the Federal Capital Territory, Abuja, Nigeria

Y. C. Adamu; S. C. Hassan; G. A. Amuga

doi:10.59573/emsj.7(4).2023.30

Автор(и)

Y. C. Adamu
S. C. Hassan
G. A. Amuga

DOI:

https://doi.org/10.59573/emsj.7(4).2023.30

Ключові слова:

Anopheles species, malaria, molecular characterization, vector

Анотація

Malaria remains a major health concern in the tropics with its vector; the Anopheles species assume several dimensions of resisting control measures. Molecular characterization of the malaria vectors was carried out in two locations of the Federal Capital Territory, Abuja Nigeria. Indoor and outdoor biting mosquitoes were collected and identified morphologically. A total of two hundred and twenty-eight (228) Anopheles mosquitoes were caught from two communities (Kubwa and Nyanya). Molecular characterization of sibling species of the An. gambiae complex was done using Polymerase Chain Reaction (PCR) techniques. The PCR molecular analysis showed that the S-form of Anopheles gambiae s.s was more abundant (76.8%) than the M-form (8.0%). The S-form occurred throughout the year with its peak at the beginning of the wet season while the M-form, An. coluzii occurred towards the end of the dry seasons. This suggests the likelihood of continuous malaria transmission all round the year. Inhabitants of the study area there have the need for effective vector control.

Посилання

Adamu, Y.C., Ruth, J.O., Tongjura, J.D. & Amuga, G.A. (2023) Distribution and abundance of Anopheles mosquitoes in two peri-urban settlements of the Federal Capital Territory, Abuja, Nigeria. International Sustainable Development Review Journal, 1(4), 189-207.

Adeleke, M. A. Mafiana, C. E. Idowu, A. B. Sam-Wobo S. O. & Idowu, O. A. (2010). Population dynamics of indoor sampled mosquitoes and their implication in disease transmission in Abeokuta, South-western Nigeria. Journal of Vector Borne Diseases, 47, 33-38.

Afolabi, O.J. Simon-Oke I.A. & Osomo, B.O. (2013). Distribution, abundance and diversity of mosquitoes in Akure, Ondo State, Nigeria. Journal of Parasitology and Vector Biology, 5(10), 132-136.

Ahmed, U. A. & Ahmed, M. M, (2011). Morphological identification of malaria vectors within Anopheles species in parts of Kano state, Nigeria. Bayero Journal of Pure and Applied Sciences, 4(2), 160-163.

Awolola, T. S., Oyewole, I. O., Koekemoer, L. L., & Coetzee, M. (2005). Identification of three members of the An. funestus group and their role in transmission in two ecological zones of Nigeria. Transactions of the Royal Society of Tropical Medicine and Hygiene, 99(7), 25-531.

Coetzee, M. (2004). Distribution of the African malaria vectors of the Anopheles gambiae complex. American Journal of Tropical Medicine and Hygiene, 70, 103–104.

Coetzee, M. Craig M. & le Sueur, D. (2000). Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitology Today, 16, 74–77.

Cuamba, N., Choi, K. S., & Townson, H. (2006). Malaria vectors in Angola: distribution of species and molecular forms of the Anopheles gambiae complex, their pyrethroid insecticide knockdown resistance (KDR) status and Plasmodium falciparum sporozoite rates. Malaria Journal, 5, 2-10.

Gillet, J.D. (1972). Common African Mosquitoes and their Medical Importance. London: William Heinemann Medical Books Limited.

Gillies M. T. & De Meillon, B. (1968). The Anophelinae of Africa, South of Sahara, Johanesburg. South Africa Institute of Medical Research, 54, 343-344.

Hougard, J.M. Fontenille, D. Chandre, F. Darriet, F. Carnevale, P. Guillet, P. (2002). Combating Malaria vectors in Africa: Current directions of research. Trends in Parasitology, 18, 283-286.

Inyang, A.A. & Mfonobong, E.A. (2016). Morphological and molecular characterisation of Anopheles mosquitoes and their behavioural patterns in Uyo, Nigeria. International Journal of Tropical Disease & Health, 14(2), 1-11.

Mosquito World. (2020). Mosquito. Retrieved February 2, 2020, from www.mosquitoworld.net.

Moreno, M. Cano, J. Nzambo, S. Bobuakasi, L. Buatiche J.N. & Ondo, M. (2004). Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea. Malaria Journal, 3, 6-7.

Okoye, C.O. & Nwachukwu, M.C. (2014). Human Induced Environmental Factors and Mosquito Breeding in Enugu State Urban-Nigeria. American Journal of Engineering and Research (AJER), 3(5), 57-63.

Onyido, A.E., Ezeani, A.C., Irikannu, K.C., Umeaneto, P.U., Egbuche, C.M., & Chikezie, F.M. (2016). Anthropophilic mosquito species prevalence in Nibo community, Awka South Local Government Area, Anambra State, Southeastern Nigeria. Ewemen Journal of Epidemiology and Clinical Medicine, 2(1), 14-20.

Service, M. W. (1985). A guide to Medical Entomology (J. Grant, Ed.). Macmillian Tropical and Sub Tropical Medical Texts, The Macmillan Press Ltd.

Souza, D., Kelly-Hope, L., Lawson, B., Wilson, M., & Boakye, D. (2010). Environmental Factors Associated with the Distribution of Anopheles gambiae s.s in Ghana; an Important Vector of Lymphatic Filariasis and Malaria. PLoS One, 5(3), 9927.

Touristlink.com. (2013). Abuja Nigeria Tourist Information. Retrieved on 30 August, 2018.

White, G. B. (1985). Anopheles bwambae sp. a malaria vector in Semliki Valley, Uganda, and its relationships with other sibling species of the An. gambiae complex. Systematic Entomology, 10, 501-522.

World Bank Group. (2015). Malaria. Retrieved January 23, 2020, from www.worldbank.org.

Yohannes, M. & Boelee, E. (2012). Early biting rythms in the afro-tropical vectors of malaria, Anopheles arabiensis and challenges for its control in Ethiopia. Medical and veterinary Entomology, 26, 103–105.